The application of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method in microbial screening

Denaturing Gradient Gel Electrophoresis (DGGE) in Microbial Ecology – Insights from Freshwaters

Although freshwater ecosystems support a great diversity of life, knowledge of its total diversity is far to be complete, particularly among microbes (Dudgeon et al., 2006). Aquatic microorganisms are pivotal in several biogeochemical processes, playing a key role in the recycling of organic material, or contributing to energy transfer in food webs, since they constitute food sources to micro-f...

Bacterial analysis of combined periodontal-endodontic lesions by polymerase chain reaction-denaturing gradient gel electrophoresis.

We used denaturing gradient gel electrophoresis (DGGE) to compare bacterial profiles in periodontium and root canals of teeth with combined periodontal-endodontic lesions. Samples of dental plaque and necrotic pulp were collected from thirteen extracted teeth with advanced periodontitis. Genomic DNA was extracted for polymerase chain reaction (PCR) analysis using universal bacterial primers. Th...

Application of Nested PCR-DGGE (Denaturing Gradient Gel Electrophoresis) for the Analysis of Ciliate Communities in Soils

Ciliates play important roles as prey and predators in ecosystems. Changes in the ciliate community can affect the composition and population of microfauna and microflora in ecosystems. To investigate the structure of ciliate communities, we developed a nested PCR-DGGE method, which combines a universal eukaryotic-specific primer set in the first PCR step with a ciliate-specific primer set in t...

Microbial Community Fingerprints by Differential Display Denaturing Gradient 1 Gel Electrophoresis ( DD - DGGE )

Application of PCR-Denaturing-Gradient Gel Electrophoresis (DGGE) Method to Examine Microbial Community Structure in Asparagus Fields with Growth Inhibition due to Continuous Cropping

Growth inhibition due to continuous cropping of asparagus is a major problem; the yield of asparagus in replanted fields is low compared to that in new fields, and missing plants occur among young seedlings. Although soil-borne disease and allelochemicals are considered to be involved in this effect, this is still controversial. We aimed to develop a technique for the biological field diagnosis...